LOCUS       sequence-71231-e        2240 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides 3xmCherry as GreenGate coding sequence module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGC026
FEATURES             Location/Qualifiers
     source          1..2240
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..710
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(148..166)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     416..435
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
       61 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      121 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
      181 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
      241 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      301 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      361 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
      421 taatgcagaa gaagacgatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
      481 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
      541 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
      601 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
      661 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtacctctg
      721 gatctggatc aggttctggt agtggttctg gttcaggatc tgcatgcatg gtttctaagg
      781 gtgaagagga taacatggct atcatcaaag agttcatgag gttcaaggtg cacatggaag
      841 gatctgttaa cggacatgag ttcgagattg aaggtgaagg tgagggtaga ccttatgagg
      901 gaactcaaac tgctaagctc aaggttacaa agggtggacc tcttcctttc gcttgggata
      961 ttctttcacc tcagttcatg tacggatcta aggcttacgt taagcaccct gctgatatcc
     1021 ctgattacct caagttgtct ttcccagagg gattcaagtg ggagagagtt atgaatttcg
     1081 aggatggtgg tgtggtgact gtgactcaag attcttcact tcaggatggt gagttcatct
     1141 acaaggtgaa gctcagggga actaacttcc cttctgatgg acctgtgatg cagaaaaaga
     1201 ctatgggatg ggaagcttct tctgagagaa tgtatcctga agatggtgct ctcaagggtg
     1261 agatcaagca gagacttaag ctcaaagatg gtggacacta cgatgctgag gttaagacta
     1321 cttacaaggc taagaagcct gttcagcttc ctggtgctta caacgttaac atcaagctcg
     1381 atatcacctc tcacaacgag gattacacta tcgttgagca gtatgagaga gctgagggaa
     1441 ggcattctac tggtggaatg gatgaacttt acaagccgcg gtctggaagt ggtagtggaa
     1501 gtggatcagg tagtggatct ggaagtaccg gtatggttag taaaggtgaa gaggataata
     1561 tggcaattat caaagaattt atgagattca aagttcatat ggaaggttca gtgaatggtc
     1621 acgagtttga aattgagggt gagggtgaag gtaggccata cgaaggtaca caaaccgcta
     1681 aacttaaggt gaccaaaggt ggtcctttgc catttgcttg ggatatcttg tctccacaat
     1741 tcatgtatgg tagtaaggca tatgtgaagc acccagcaga tattccagat tatcttaagc
     1801 tctcattccc tgaaggtttt aagtgggaaa gggtgatgaa cttcgaagat ggtggtgttg
     1861 ttaccgtgac ccaggatagt tctctccaag atggtgagtt tatctataag gttaagttga
     1921 gaggtacaaa cttcccatca gatggtccag ttatgcaaaa gaaaacaatg ggttgggagg
     1981 cttcaagtga gaggatgtac ccagaggatg gtgcacttaa aggtgaaatc aaacaaaggc
     2041 tcaagttgaa ggatggtggt cattatgatg cagaggtgaa aaccacctat aaggcaaaga
     2101 aacctgtgca attgcctggt gcatacaatg tgaatattaa gttggatatc accagtcata
     2161 atgaagatta caccattgtg gaacagtacg aaagggcaga aggtagacat tcaacaggtg
     2221 gtatggatga gttgtacaag
//
