LOCUS       sequence-71233-e        1814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides GUS as GreenGate coding sequence module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGC051
FEATURES             Location/Qualifiers
     source          1..1814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..1814
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
ORIGIN
        1 caacaatggt ccgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt
       61 gggcattcag tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt
      121 tacaagaaag ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag
      181 atattcgtaa ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt
      241 gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg
      301 tcaataatca ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca
      361 cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga
      421 actggcagac tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt
      481 cttacttcca tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca
      541 cgccgaacac ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc
      601 acgcgtctgt tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg
      661 cggatcaaca ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc
      721 cgcacctctg gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc
      781 agacagagtg tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg
      841 aacagttcct gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg
      901 cggacttgcg tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg
      961 actggattgg ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg
     1021 actgggcaga tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc
     1081 tctctttagg cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg
     1141 cagtcaacgg ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg
     1201 acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc
     1261 aaggtgcacg ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc
     1321 gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc
     1381 tctttgatgt gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg
     1441 aaacggcaga gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc
     1501 cgattatcat caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg
     1561 acatgtggag tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc
     1621 gcgtcagcgc cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag
     1681 gcatattgcg cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt
     1741 cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc
     1801 agggaggcaa acaa
//
