LOCUS       sequence-71239-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides linker:mCherry as GreenGate C-terminal tag module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGD003
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..812
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(247..265)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     515..534
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgcagcggc cgcttcaggg agtggttccg gaagcggctc tggatcgggc tcagggagtg
       61 gttccggcag cggctctgga tcggcgatcg cagctgcagc catggtgagc aagggcgagg
      121 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      181 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      241 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      301 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
      361 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
      421 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
      481 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagacgatgg
      541 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
      601 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
      661 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
      721 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
      781 ccaccggcgg catggacgag ctgtacaagt ag
//
