LOCUS       sequence-71248-e        5520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the WUS terminator as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGE002
FEATURES             Location/Qualifiers
     source          1..5520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5106..5125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5107..5125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5135..5157)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5351..5370)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5470..5492
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcactg caatagctct tacgccggtg tcgctcggga
     2281 ttaaagctct ttcctctctc tctctctttc gtactcgtat gttcacaact atgcttcgct
     2341 agtgattaat gatgcagttg ttatattagt agttaactag ttatctctcg ttatgtgtaa
     2401 tttgtaatta ctagctaagt atcgtctagg ttttaattgt aattgacaac cgttttatct
     2461 ctatgatgaa taagttaaaa ttttaattac tactatgaag aaatgactat ttcagtaaat
     2521 ttttaaaaag gaattcctca cagatccgga ggcatgaaag aaatgctaga gatgtactat
     2581 atttgattac aatgttatga tcttaattat cgattacaac gtgatcttga accgaaatca
     2641 acaatagttg ttttacaaca gaacttgtga acagttgaat gttttttggt agttcaaaac
     2701 taaaacaagc aagttgcttt tcagtggaag ttttcttctt cttcatgtta tgcaatcagc
     2761 ttgatctatc gataatcatg gaattttgat tacatcaata cgaacggtca aaatcaatct
     2821 caattatttc tatgcggttt tttcctacct aaattttgat tacatgcatt tatcaatccg
     2881 aactcacaca attttcaaat aataaatctg agttatgtaa ccgttagcca atttcaacat
     2941 atattcatta taaattaata ttaatcgtta gccaattaaa taaaaggaat gatttagata
     3001 caaaataagt gataatttta acttaacaat tcaaatcgac attaacatgt atatgttaca
     3061 cagttcagtt tgttatttaa aaattatgta agtctaggtt gataaataca gatgaccaaa
     3121 atttgaatta aaaaaagacc gcatcaaaat agttggggtt gattttggtc gttatcccca
     3181 aaaaatgcaa cctcgaaccc tttattgata agattaaact atatgggagg gtaataagtt
     3241 tataggggtt tcttctaagt tcatatttct atttaaaacc tacagcaaag taggccaaaa
     3301 tggtacaata gcaaaccatt acaattcaaa tcaaattttg ttattgataa gttagaattt
     3361 gttgttgttt aaatacttca taaatacttt tcgaaataat acaattttaa attgttaaga
     3421 aaaaagaaat aacatttatc aataaaatta agattatagt gaaaaacctt gaaccctagt
     3481 cattcaacga tctcgtttac ccttattttt tccaaaatgt ttgttatgta tactagggat
     3541 gagttaggga atgaatatcc tcgattttgg tggtatttgt gatatgctct tagttatctg
     3601 atatgtattc gttccgtaac tcatcatata tccgttaaaa tcagaagtat ttgcttaata
     3661 tataactaaa aataactata aaaaatgaaa aacatataaa aaaaactcaa aaaataaatt
     3721 caaaatagca aaattttatt aaaaatattt cgttaacttt taaaactagt agttaaataa
     3781 ataattaatt tgatgaacaa aatttataaa agttaaatat ataaagtaaa ttataatgga
     3841 attagctata tacatatgta tatatatacg agagcaaaac ggaaatcaat tttttactag
     3901 atgggttata tgatattaat ttttaatatt caatttgttt cataaaacta cagatgtcaa
     3961 aagtttttga atcgaatcta aacgaataac agatctaaat ggaactaaca aaatattttg
     4021 ccgagttggt atgtttaatt atatctgaac tatttaatga tggtcatcga tatgacgata
     4081 tgaaatgttt ttattttctc catctcgttc aaaacgacaa agttaatgat gaccatcaat
     4141 ggctgattga tcactacaat ttttttattt tttattttta acacttatat ctaaaattag
     4201 tttgtagtaa cactaatgtc cctttgtaat ctttggatac aattaagtac atatatgatt
     4261 agttataagt tcttctttag tgctatgaat tacgtcaagg tggctcacgc gttgtgagag
     4321 tgcgtgtcat tcagtatttc cttaaacaga gagatctttt acaaagagct atcctcgagc
     4381 cacgtgaact aaaatgacac aaggaagaaa aatgtttttt ttgttagttg atctatgtca
     4441 catagatctt gttcaatgac cgtagctttt gatagaattt ttttaaaaat atgttacata
     4501 ctcataaata aattatatgg gttatatttg cttataaatc ttgtacatta tactatacaa
     4561 tctgttttat tgtgaattgt gtattaagaa tcattttaac aaaaaaaaat taaaaaataa
     4621 tataatactg tatgttaaaa aaacatgaat gtggcagtta gggacatttg tgcatccatg
     4681 ttttaaagag tacatttttt gtagtttgaa ccctttcttc ctcgtaaact acgctcataa
     4741 ttgttacatt gaaaacttta atcatggagt attaactaga tatgtttttc tttggattgc
     4801 ctcgttatat gttgttcctt ctcttgttat ccaagtttct ctattatcca agttttgtcc
     4861 aaacacgaat accaacaaac tacatgtcta tatttatatt ttgaaaaaat gaaaatttaa
     4921 gtggtttagc agataaaacg aactgatgtg ttttcggcca aatccattta taattaagat
     4981 tttccatttt accattatta atttacagta atgcgtagat taaaaatatt aagttgttat
     5041 gtatcagtga tagtcttcta cttgtaagta gaaagtctac tgattactat gagaccgaat
     5101 tctcgcccta tagtgagtcg tattacctgg ccgtcgtttt acaacgtcgt gactgggaaa
     5161 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5221 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5281 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     5341 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     5401 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5461 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
//
