LOCUS       sequence-71253-e        1203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a BASTA resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF001
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..383
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             394..945
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      951..1203
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
ORIGIN
        1 gtttttcaaa tcagtgcgca agacgtgacg taagtatccg agtcagtttt tatttttcta
       61 ctaatttggt cgtttatttc ggcgtgtagg acatggcaac cgggcctgaa tttcgcgggt
      121 attctgtttc tattccaact ttttcttgat ccgcagccat taacgacttt tgaatagata
      181 cgctgacacg ccaagcctcg ctagtcaaaa gtgtaccaaa caacgcttta cagcaagaac
      241 ggaatgcgcg tgacgctcgc ggtgacgcca tttcgccttt tcagaaatgg ataaatagcc
      301 ttgcttccta ttatatcttc ccaaattacc aatacattac actagcatct gaatttcata
      361 accaatctcg atacaccaaa tcgggtacca acaatgagcc cagaacgacg cccggccgac
      421 atccgccgtg ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa ccactacatc
      481 gagacaagca cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac
      541 ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc
      601 ggcatcgcct acgcgggtcc ctggaaggca cgcaacgcct acgactggac ggccgagtcc
      661 accgtgtacg tctccccccg ccaccagcgg acgggactgg gctccacgct ctacacccac
      721 ctgctgaagt ccctggaggc acagggcttc aagagcgtgg tcgctgtcat cgggctgccc
      781 aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg
      841 gcggccggct tcaagcacgg gaactggcat gacgtgggtt tctggcagct ggacttcagc
      901 ctgccggtgc cgccccgtcc ggtcctgccc gtcaccgaaa tctgagagct cttggactcc
      961 catgttggca aaggcaacca aacaaacaat gaatgatccg ctcctgcata tggggcggtt
     1021 tgagtatttc aactgccatt tgggctgaat tgaagacatg ctcctgtcag aaattccgtg
     1081 atcttactca atattcagta atctcggcca atatcctaaa tgtgcgtggc tttatctgtc
     1141 tttgtattgt ttcatcaatt catgtaacgt ttgcttttct tatgaatttt caaataaatt
     1201 atc
//
