LOCUS       sequence-71255-e        1628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a BASTA resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF002
FEATURES             Location/Qualifiers
     source          1..1628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        496..841
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     784..804
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             860..1411
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 gtgtccccag attagccttt tcaatttcag aaagaatgct aacccacaga tggttagaga
       61 ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc aggaaatcaa
      121 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      181 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      241 cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac
      301 tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa
      361 gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt
      421 caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga
      481 agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt
      541 ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta
      601 caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg
      661 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      721 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      781 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac
      841 acgggggacg gtaccaacaa tgagcccaga acgacgcccg gccgacatcc gccgtgccac
      901 cgaggcggac atgccggcgg tctgcaccat cgtcaaccac tacatcgaga caagcacggt
      961 caacttccgt accgagccgc aggaaccgca ggagtggacg gacgacctcg tccgtctgcg
     1021 ggagcgctat ccctggctcg tcgccgaggt ggacggcgag gtcgccggca tcgcctacgc
     1081 gggtccctgg aaggcacgca acgcctacga ctggacggcc gagtccaccg tatacgtctc
     1141 cccccgccac cagcggacgg gactgggctc cacgctctac acccacctgc tgaagtccct
     1201 ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg ctgcccaacg acccgagcgt
     1261 gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg ctgcgggcgg ccggcttcaa
     1321 gcacgggaac tggcatgacg tgggtttctg gcagctggac ttcagcctgc cggtgccgcc
     1381 ccgtccggtc ctgcccgtca ccgaaatctg agagctcagt ccgcaaaaat caccagtctc
     1441 tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat
     1501 aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
     1561 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     1621 tccagtga
//
