LOCUS       sequence-71259-e        2183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a D-alanine resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF004
FEATURES             Location/Qualifiers
     source          1..2183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        496..841
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     784..804
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gtgtccccag attagccttt tcaatttcag aaagaatgct aacccacaga tggttagaga
       61 ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc aggaaatcaa
      121 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      181 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      241 cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac
      301 tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa
      361 gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt
      421 caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga
      481 agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt
      541 ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta
      601 caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg
      661 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      721 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      781 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac
      841 acgggggacg gtaccaacaa tgcactcgca gaagcgcgtc gttgtcctcg gatcaggcgt
      901 tatcggtctg agcagcgccc tcatcctcgc tcggaagggc tacagcgtgc atattctcgc
      961 gcgcgacttg ccggaggacg tgtcgagcca gactttcgct tcaccttggg ctggcgcgaa
     1021 ttggacgcct ttcatgacgc ttacagacgg tcctcgacaa gcaaaatggg aagaatcgac
     1081 tttcaagaag tgggtcgagt tggtcccgac gggccatgcc atgtggctca aggggacgag
     1141 gcggttcgcg cagaacgaag acggcttgct cgggcactgg tacaaggaca tcacgccaaa
     1201 ttaccgcccc ctcccatctt ccgaatgtcc acctggcgct atcggcgtaa cctacgacac
     1261 cctctccgtc cacgcaccaa agtactgcca gtaccttgca agagagctac agaagctcgg
     1321 cgcgacgttt gagagacgga ccgttacgtc gcttgagcag gcgttcgacg gtgcggattt
     1381 ggtggtcaac gctacgggac ttggcgccaa gtcgattgcg ggcatcgacg accaagccgc
     1441 cgagccaatc cgcgggcaaa ccgtcctcgt caagtcccca tgcaagcgat gcacgatgga
     1501 ctcgtccgac cccgcttctc ccgcctacat cattccccga ccaggtggcg aagtcatctg
     1561 cggcgggacg tacggcgtgg gagactggga cttgtctgtc aacccagaga cggtccagcg
     1621 catcctcaag cactgcttgc gcctcgaccc gaccatctcg tcggacggaa cgatcgaagg
     1681 catcgaggtc ctccgccaca acgtcggctt gcgacctgca cgacgaggcg gaccccgcgt
     1741 tgaggcagaa cggatcgtcc tgcctctcga ccggacaaag tcgcccctct cgctcggcag
     1801 gggcagcgca cgagcggcga aggagaagga ggtcacgctt gtgcatgcgt atggcttctc
     1861 cagtgcggga taccagcaga gttggggcgc ggcggaggat gtcgcgcagc tcgtggacga
     1921 ggcgttccag cgctaccacg gcgcggcgcg ggagtcgaag ttgtaggagc tcagtccgca
     1981 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt
     2041 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat
     2101 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     2161 ttcctaaaac caaaatccag tga
//
