LOCUS       sequence-71263-e        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a kanamycin resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF007
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        73..252
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             286..1080
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(340..359)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     950..969
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt
       61 ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt ctggagttta
      121 atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac
      181 tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc
      241 ggtatccaat tagagtctca tattcactct caactcgatc gaggcatgat tgaacaagat
      301 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
      361 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
      421 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg
      481 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      541 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      601 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
      661 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
      721 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
      781 gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc
      841 gtgacccacg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
      901 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
      961 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     1021 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     1081 gcgggactct ggggttcgga ctctagctag agtcaagcag atcgttcaaa catttggcaa
     1141 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     1201 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     1261 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     1321 cgcgcaaact aggataaa
//
