LOCUS       sequence-71265-e        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a BASTA resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF008
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        73..252
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             294..845
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt
       61 ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt ctggagttta
      121 atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac
      181 tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc
      241 ggtatccaat tagagtctca tattcactct caactcgatc gaggggatct accatgagcc
      301 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
      361 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
      421 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
      481 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggtcc ctggaaggca cgcaacgcct
      541 acgactggac ggccgagtcc accgtgtacg tctccccccg ccatcagcgg acgggactgg
      601 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
      661 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
      721 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
      781 tctggcagct ggacttcagc ctgccggtgc cgccccgtcc ggtcctgccc gtcaccgaaa
      841 tctgatgacc cctagagtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga
      901 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
      961 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     1021 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     1081 aaa
//
