LOCUS       sequence-7128-ex        2597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Cre recombinase driven by the human 
            cytomegalovirus IE promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Sauer B, Henderson N
  TITLE     Targeted insertion of exogenous DNA into the eukaryotic genome by 
            the Cre recombinase.
  JOURNAL   New Biol. 1990 May . 2(5):441-9.
  PUBMED    2288914
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Biol. 
            1990 May . 2(5):441-9."
COMMENT     Sequence Label: pBS185 CMV-Cre
FEATURES             Location/Qualifiers
     source          1..2597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..1089
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
ORIGIN
        1 ctcgaggggc agagccgatc ctgtacactt tacttaaaac cattatctga gtgtgaaatg
       61 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
      121 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
      181 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
      241 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
      301 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
      361 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
      421 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
      481 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
      541 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
      601 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
      661 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
      721 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
      781 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
      841 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
      901 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
      961 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
     1021 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
     1081 ggcgattagc cattaacgcg taaatgattg ctataattat ttgatattta tggtgacata
     1141 tgagaaagga tttcaacatc gacggaaaat atgtagtgct gtctgtaagc actaatattc
     1201 agtcgccagc cgtcattgtc actgtaaagc tgagcgatag aatgcctgat attgactcaa
     1261 tatccgttgc gtttcctgtc aaaagtatgc gtagtgctga acatttcgtg atgaatgcca
     1321 ccgaggaaga agcacggcgc ggttttgcta aagtgatgtc tgagtttggc gaactcttgg
     1381 gtaaggttgg aattgtcgag ggatctcgga atggacccca actgctcctg ctccaccggt
     1441 aagactcccg atccttggtc tttagaatac caagttggga ccgcagagcg gaatccccga
     1501 gttgtagagg cttggcggga ataggcacct ttagttggcg attcattccg gttctttcta
     1561 gaatccgctc ttgcaaaagc cttcattagt tacgagtatt gtcgaacggg tcctttggcg
     1621 gggttggggc taggatttag acgcgcaaat gtccggttcc tgatcaccca gttagtgggg
     1681 acatctgggt tgagtcccag gcattactaa acttactgtg aattgcttga attaagaaag
     1741 aggtgaagga cctttatgtc ttgggactca aagacataat ccctgactta acctgtgagg
     1801 agaaaagtgg ggctaggctc cctgcagctc cgaggaggac ttagtgaact gagccgggac
     1861 tcgtggtgtt ggccactgct gtaatgctgc ctccctcatg ctgtcttctt tctcctccca
     1921 ggcggctcct gcacttgcac cagctcctgc gcctgcaaga actgcaagtg cacctcctgc
     1981 aagaagagtg agttgggaca ccttgggtgg cggctaaggc taggggcggg gaactcctac
     2041 aaaactggct ctgagaaatg tcctttgctt cccggaggcc attgtattgt ctcggggaca
     2101 gaactataca gagaactatt taaaaaaacc gaggtcttct ctgttgggga caggaagcag
     2161 aggtcttcag ccaggctgcc tcttcctcct tcttctaggc tgctgctcct gctgtcccgt
     2221 gggctgctcc aaatgtgccc agggctgtgt ctgcaaaggc gccgcggaca agtgcacgtg
     2281 ctgtgcctga tgtgacgaac agcgctgcca ccacgtgtaa atagtatcgg accaacccag
     2341 cgtcttccta tacagttcca ccctgtttac taaacccccg ttttctaccg agtacgttaa
     2401 taataaaagc ctgtttgagt ctaactctgg tttcttggtg tggtttggca ataagaaact
     2461 ggggtgactt gatagtctgg ggatctggtt ttggaccccc tcgtgccttt acctccgccc
     2521 tctggccctc acagaggggt aatgtctttg ggtaaagcca agctatatcc cataagcttc
     2581 ctcatggaaa acagctg
//
