LOCUS       sequence-7129-ex        1870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre-GFP fusion under the control of the Rous sarcoma virus
            (RSV) LTR/promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Gagneten S, Le Y, Miller J, Sauer B
  TITLE     Brief expression of a GFP cre fusion gene in embryonic stem cells 
            allows rapid retrieval of site-specific genomic deletions.
  JOURNAL   Nucleic Acids Res. 1997 Aug 15. 25(16):3326-31.
  PUBMED    9241248
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 1997 Aug 15. 25(16):3326-31."
COMMENT     Sequence Label: pBS448 RSV-GFPcre
FEATURES             Location/Qualifiers
     source          1..1870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..731
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(48..76)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     671..691
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             813..1844
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
ORIGIN
        1 aagcttggtc gagtgccatg agtaaaggag aagaactttt cactggagtt gtcccaattc
       61 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      121 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      181 ttccttggcc aacacttgtc actactttca cttatggtgt tcaatgcttt tcaagatacc
      241 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      301 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      361 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      421 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      481 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      541 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      601 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      661 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      721 aactatacaa aggcgccggc gctggtgctg gtgctggcgc catcatggca ctcgacggag
      781 aaagctcagg ctctggctca gagtctgact ccatggccaa tttactgacc gtacaccaaa
      841 atttgcctgc attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca
      901 tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc
      961 ggtcgtgggc ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag
     1021 atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc
     1081 aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca
     1141 gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac
     1201 gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg
     1261 aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca
     1321 ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg
     1381 gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag
     1441 agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg
     1501 tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc
     1561 catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc
     1621 gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca
     1681 gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca
     1741 tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata
     1801 gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga ttagccatta acgcgtaaat
     1861 gattgctcta
//
