LOCUS       sequence-71293-e        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate N-terminal tag + coding 
            sequence + C-terminal tag + terminator modules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGH000
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..710
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(117..136)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             729..1034
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     913..932
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ggtctcaaac aaaggatcca gtgctaacat ggtctagaag gaggtcagct atggagaaaa
       61 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      121 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      181 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      241 cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt gagctggtga
      301 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
      361 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
      421 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
      481 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
      541 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
      601 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
      661 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa gagctcagga
      721 ggacagctat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
      781 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
      841 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccagtggtg catatcgggg
      901 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccagtctcc gttatcgggg
      961 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1021 tctggggaat ataactgcag aggaggtaat caatgccaca acactggtgg taccactatg
     1081 agacc
//
