LOCUS       sequence-71296-e        3478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate supermodules w/o plant 
            resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGM000
FEATURES             Location/Qualifiers
     source          1..3478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     CDS             499..1158
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(565..584)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1177..1482
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1361..1380
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(1512..1534)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1728..1747)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1918..1937)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1929..2738
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1996..2015)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2828..3416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3317..3336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggcccggt
      361 ctcaacctga attcgcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
      421 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct
      481 aagcttagga ggtcagctat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
      541 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
      601 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
      661 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggagttccgt
      721 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
      781 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
      841 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
      901 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
      961 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     1021 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     1081 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     1141 tggcagggcg gggcgtaaga gctcaggagg acagctatgc agtttaaggt ttacacctat
     1201 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     1261 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     1321 gaactttacc cagtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     1381 gccagtgtgc cagtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     1441 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaactagtag gaggtaatca
     1501 atgctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1561 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1621 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     1681 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     1741 gatgccgcat gatcatctgg cggacctctt cggaggtccg cttttttttc tcgagtaggt
     1801 gagaccccat ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1861 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1921 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1981 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     2041 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     2101 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2161 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2221 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2281 ttgttgatgc gctggcagtg tccctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2341 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2401 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2461 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2521 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2581 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2641 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2701 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2761 aacattattc agattgggcc ccgttccact gagcgtcaga cccggtagaa aagatcaaag
     2821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2941 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
