LOCUS       sequence-71299-e        1449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate supermodules with plant 
            resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGN000
FEATURES             Location/Qualifiers
     source          1..1449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             142..801
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(208..227)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             820..1125
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1004..1023
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(1155..1177)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1371..1390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggtctcatag ggaattcgct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
       61 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt
      121 gctaagctta ggaggtcagc tatggagaaa aaaatcactg gatataccac cgttgatata
      181 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      241 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
      301 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggagttc
      361 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
      421 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      481 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      541 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      601 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      661 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      721 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      781 gagtggcagg gcggggcgta agagctcagg aggacagcta tgcagtttaa ggtttacacc
      841 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
      901 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
      961 cgtgaacttt acccagtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     1021 atggccagtg tgccagtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     1081 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaactag taggaggtaa
     1141 tcaatgctgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1201 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1261 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     1321 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     1381 tctgatgccg catgatcatc tggcggacct cttcggaggt ccgctttttt ttctcgaggt
     1441 attgagacc
//
