LOCUS       sequence-71305-e        1654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty destination vector for creating a GreenGate plant 
            transformation plasmid with the plant resistance cassette at the 
            T-DNA right border..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGZ001
FEATURES             Location/Qualifiers
     source          1..1654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..28
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             253..912
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(319..338)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             931..1236
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1115..1134
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(1266..1288)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1482..1501)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    1605..1629
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gatcttggca ggatatattg tggtgtaacg ttctcgaact aggctcggac gaagtaagcg
       61 gccgcaacct taattaaaca cggcgcgcca acagcgatcg caccaggtac caccttgaga
      121 ccgaattcgc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
      181 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg tgctaagctt
      241 aggaggtcag ctatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      301 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      361 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      421 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggagtt ccgtatggca
      481 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      541 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      601 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      661 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      721 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      781 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      841 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      901 ggcggggcgt aagagctcag gaggacagct atgcagttta aggtttacac ctataaaaga
      961 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
     1021 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     1081 tacccagtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     1141 gtgccagtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     1201 aaaaacgcca ttaacctgat gttctgggga atataaacta gtaggaggta atcaatgctg
     1261 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     1321 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     1381 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     1441 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     1501 gcatgatcat ctggcggacc tcttcggagg tccgcttttt tttctcgagg gtctcagtat
     1561 tctagaccaa gcatgcacag ctggcgttct cagccagatc cccttgacag gatatattgg
     1621 cgggtaaact aagtcgctgt atgtgtttgt ttga
//
