LOCUS       sequence-71314-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tetO-GFP reporter casette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pCH001
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(124..579)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(261..282)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     473..492
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     705..727
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     719..736
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     720..736
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     743..762
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        743..761
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
       61 agttattacc ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
      121 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
      181 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
      241 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
      301 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
      361 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
      421 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
      481 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
      541 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
      601 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      661 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      721 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg
      781 cggtggcggc cgctctagat gcgtacacgc gtccgtacag aaaaaaaaga aaaatttgaa
      841 atataaataa cgttcttaat actaacataa ctataagaaa ataaataggg acctagactt
      901 caggttgtct aactccttcc ttttcggt
//
