LOCUS       sequence-71316-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J
  TITLE     Efficient and specific gene knockdown by small interfering RNAs 
            produced in bacteria.
  JOURNAL   Nat Biotechnol. 2013 Apr;31(4):350-6. doi: 10.1038/nbt.2537. Epub 
            2013 Mar 10.
  PUBMED    23475073
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2537"; 
            journalName: "Nat Biotechnol"; date: "2013-04"; volume: "31"; issue:
            "4"; pages: "350-6"
COMMENT     Sequence Label: pGEX-4T-1-p19-T7
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(551..568)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(595..617)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 agtcgtatta gattcagtgg tggtggtggt ggtgctcgct ttctttttcg aaggtttgag
       61 taccttcagg gcatcctctt gatacattac tttccacttc gattggggca agctgtagca
      121 gttcttgctt agaccgaatt gccatctcac agagatgctg aagagttcgc gaccctccag
      181 aaacggtgat actaactcct cgaaaccgaa tactataggt acatccgatc tggtcgaaac
      241 cgaaaaatcg agatgctgca tagttaaccg aatctcccgt ccaagatcca aggactctgt
      301 gcagtgaagc ttccgtcctg tcgtatctga gatatctctt aaatacaact ttcccgaaac
      361 cccagctttc cttgaaacca aggggattat cttgattcga attcgtctca tcgttatgta
      421 gccgccactc agtccaactc ggactttcgt caggaagttt gaagggagaa gtggtacctc
      481 ctgatcctcc atcccaacgt tcactgttag cttgttccct agcgtcgttt ccttgtatag
      541 ctcgttccat ggatccacgc ggaaccagat ccgattttgg aggatggtcg ccaccaccaa
      601 acgtggcttg ccagccctgc aaaggccatg ctatatactt gctggatttc aagtacttat
      661 caatttgtgg gatagcttca atacgttttt taaaacaaac taattttggg aacgcatcca
      721 ggcacattgg gtccatgtat aaaacaacat caagagcgtc atacaacatg aagtcaggat
      781 gggttacatg atcaccattt aaatatgttt tatgacataa acgatcttcg aacattttca
      841 gcatttcagg tagcttgcta agaaaatcaa ctttgagagt tcaagcttac aagcat
//
