LOCUS       sequence-71318-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Bossuyt J, Chang CW, Helmstadter K, Kunkel MT, Newton AC, Campbell 
            KS, Martin JL, Bossuyt S, Robia SL, Bers DM
  TITLE     Spatiotemporally distinct protein kinase D activation in adult 
            cardiomyocytes in response to phenylephrine and endothelin.
  JOURNAL   J Biol Chem. 2011 Sep 23;286(38):33390-400. doi: 
            10.1074/jbc.M111.246447. Epub 2011 Jul 27.
  PUBMED    21795686
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.246447"; journalName: "J Biol Chem"; date: 
            "2011-09-23- 23"; volume: "286"; issue: "38"; pages: "33390-400"
COMMENT     Sequence Label: pcDNA3-DKAR-Nuc
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(93..95,96..98,99..809)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(93..95,96..98,99..809)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(93..95,96..98,99..809)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(138..159)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(399..418)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     746..767
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcagagct ctctggctac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttcgcca ccatggtgag caagggcgag gagctgttca
      121 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aggttcagcg
      181 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      241 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc
      301 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      361 ccgaaggcta cgtccaggag cgtaccatct tcttcaagga cgacggcaac tacaagaccc
      421 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      481 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca
      541 acgtctatat caccgccgac aagcagaaga acggcatcaa ggcccacttc aagatccgcc
      601 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      661 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      721 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      781 tcactcacgg catggacgag ctgtacaagg ctggtaccaa gggtaatggt
//
