LOCUS       sequence-71320-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Bossuyt J, Chang CW, Helmstadter K, Kunkel MT, Newton AC, Campbell 
            KS, Martin JL, Bossuyt S, Robia SL, Bers DM
  TITLE     Spatiotemporally distinct protein kinase D activation in adult 
            cardiomyocytes in response to phenylephrine and endothelin.
  JOURNAL   J Biol Chem. 2011 Sep 23;286(38):33390-400. doi: 
            10.1074/jbc.M111.246447. Epub 2011 Jul 27.
  PUBMED    21795686
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.246447"; journalName: "J Biol Chem"; date: 
            "2011-09-23- 23"; volume: "286"; issue: "38"; pages: "33390-400"
COMMENT     Sequence Label: pcDNA3-DKAR-Nuc
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(23..733,734..736))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(65..86)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     414..433
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     673..694
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctcactttcg cttcttcttg ggcttgtaca gctcgtccat gccgtgagtg atcccggcgg
       61 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct
      121 tggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      181 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
      241 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
      301 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaaa tcgatgccct
      361 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
      421 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
      481 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac atcaggccgt
      541 agccgaaggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
      601 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
      661 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
      721 cctcgccctt gctcactcca ccagatccac cttcggagac agctgcagtc agttgcctcg
      781 acagacctcc ggatcctcct aactttttca ccaaatcttt ttcttcggct gtttgcttaa
      841 gtactactct ttgttcttgt aacataccta atccctcgtt
//
