LOCUS       sequence-71331-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_TGAA
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..19
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3..19
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttgtaaaacg acggccagtc ttaagcgtct catggcctga ccccggacca agtggtggct
       61 atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
      121 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc
      181 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
      241 ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa
      301 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
      361 gtggctatcg ccagcaacat tggcggcaag caagcgctcg aaacggtgca gcggctgttg
      421 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgagacgtct
      481 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
      541 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
      601 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      661 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
      721 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      781 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
      841 gggaagcggt gatcgccgaa
//
