LOCUS       sequence-71348-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z
  TITLE     A broad-range of recombination cloning vectors in mycobacteria.
  JOURNAL   Plasmid. 2009 Nov . 62(3):158-65.
  PUBMED    19647016
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid. 
            2009 Nov . 62(3):158-65."
COMMENT     Sequence Label: pJAK2.B
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(125..249)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      318..364
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(514..533)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(598..617)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
       61 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
      121 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
      181 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
      241 caaagtggtt gatctgcaga attcgaagct tatcgatgtc gacgtagtta actagcgtac
      301 gatcgactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      361 ttatgccatc atggccgcgg tgatcagcta gccacctgac gtcggggggg ggggaaagcc
      421 acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
      481 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
      541 gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
      601 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
      661 agccccatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
      721 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
      781 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag
      841 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
      901 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
      961 tatttcgtct cgctcaggcg caatc
//
