LOCUS       sequence-71364-e        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen lentivector expressing shRNA from H1 promoter and GFP. For 
            direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from 
            pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Wiznerowicz M, Trono D
  TITLE     Conditional suppression of cellular genes: lentivirus 
            vector-mediated drug-inducible RNA interference.
  JOURNAL   J Virol. 2003 Aug . 77(16):8957-61.
  PUBMED    12885912
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2003 Aug . 77(16):8957-61."
COMMENT     Sequence Label: pLVTHM
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             6..186
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    206..239
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(274..488)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(334..353)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    join(517..535,536..558,559..577,578..600,601..619,620..642,
                     643..661,662..684,685..703,704..726,727..745,746..768,
                     769..787)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gatgctgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
       61 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
      121 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
      181 agacccagta caagcaaaaa gcagcataac ttcgtataat gtatgctata cgaagttatt
      241 cccatgcgac ggtatcgata agcttggaga cgcgtggtct catacagaac ttataagatt
      301 cccaaatcca aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa
      361 atattgcagg gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc
      421 gctgggtgtt cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt
      481 cagcgttcga attcctgcag ccccgacttt cacttttctc tatcactgat agggagtggt
      541 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
      601 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
      661 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
      721 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
      781 atagggagtg gtaaactcga gggggatcca ctagcatggt gaattgatcc catcttgtct
      841 tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc
      901 tacagctgcc ttgtaagtca ttggtcttaa aggtacctgg ccatacgtac cgatgcgggg
      961 aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaa
//
