LOCUS       sequence-71374-e        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6 TEV Ade S. cerevisiae expression ...
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        212..876
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    428..545
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     840..863
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             910..927
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             937..957
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggcctcccct ggaggtcgac
       61 caattcgaca ggttatcagc aacaacacag tcatatccat tctcaattag ctctaccaca
      121 gtgtgtgaac caatgtatcc agcaccacct gtaaccaaaa caattttaga agtactttca
      181 ctttgtaact gagctgtcat ttatattgaa ttttcaaaaa ttcttacttt ttttttggat
      241 ggacgcaaag aagtttaata atcatattac atggcattac caccatatac atatccatat
      301 acatatccat atctaatctt acttatatgt tgtggaaatg taaagagccc cattatctta
      361 gcctaaaaaa accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat
      421 tgaagtacgg attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc
      481 gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg
      541 ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc
      601 agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata
      661 atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg
      721 atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac
      781 attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta
      841 atatacctct atactttaac gtcaaggaga aaaaaccccg gatccgtaac catgtcaact
      901 ggttcttctc accatcacca tcaccatggt tcttctgaaa acctgtactt ccaatccaat
      961 attggaagtg gataactctc gaggatcgta atgactagat tatataatca gaaatagcct
     1021 ttggtgatca cttccatttt agtcagcata atagaaagtt taattacgcg cttttttaca
     1081 ttccgacaaa gatctgcccg a
//
