LOCUS       sequence-71434-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated partial RH4 ectodomain with
            no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RH4-bio
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     501..520
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      543..552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtttagtgaa ccgtcagatc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
       61 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      121 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      181 tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag gctgagcacc
      241 gtggcgggcg gcagcgggtg gcggtcgggg ttgtttctgg cggaggtgct gctgatgatg
      301 taattaaagt aggcggtctt gagacggcgg atggtcgagg tgaggtgtgg caggcttgag
      361 atccagctgt tggggtgagt actccctctc aaaagcgggc attacttctg cgctaagatt
      421 gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgatctgg ccatacactt
      481 gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaagt
      541 ttgccaccat ggagtttcag acccaggtac tcatgtccct gctgctctgc atgtctggtg
      601 cggccgccat ccccagcaaa gagaagaaga acgaccccga ggccgacagc aagaacagcc
      661 agaaccagca cgacatcaac aaggcccacc acaccaacaa caactacgac ctgaacatca
      721 aggacaagga cgagaagaag cggaagaacg acaacctgat caacaattac gactacagcc
      781 tgctgaagct gtcctacaac aagaaccagg acatctacaa gaatatccag aacggccaga
      841 agctgaaaac cgacatcatc ctgaacagct tcgtgcagat caacagcgcc aacatcctga
      901 tggacgagat cgagaactac gtgaa
//
