LOCUS       sequence-71439-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase Reporter for mutant STAT3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Dabir S, Kluge A, Dowlati A
  TITLE     The association and nuclear translocation of the PIAS3-STAT3 complex
            is ligand and time dependent.
  JOURNAL   Mol Cancer Res. 2009 Nov;7(11):1854-60. doi: 
            10.1158/1541-7786.MCR-09-0313. Epub 2009 Nov 10.
  PUBMED    19903771
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1541-7786.MCR-09-0313"; journalName: "Mol Cancer Res"; 
            date: "2009-11"; volume: "7"; issue: "11"; pages: "1854-60"
COMMENT     Sequence Label: STAT3(Y705F)-TAL-Luc
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(91..122)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             complement(216..239)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctttcttta tgtttttggc gtcttccatg gtggctttac caacagtacc ggaatgccaa
       61 gctttttacc aacagtaccg gaatgccaag ctggaattcg agcttccatt atataccctc
      121 tagagtctag atctcgagcg gccgccactg tgctggatat ctgcagaatt ccaccacact
      181 ggactagtgg atccgagctc ggtaccaagc tttcacttgt catcgtcgtc cttgtagtcc
      241 atgggggagg tagcgcactc cgaggtcaac tccatgtcaa aggtgaggga ctcaaactgc
      301 cctcctgctg agggttcagc accttcacca ttatttccaa actgcatcaa tgaatctaaa
      361 gtgcgggggg acatcggcag gtcaatggta ttgctgcagg tcgttggtgt cacacagata
      421 aacttggtct tcaggaatgg ggcagcgcta cctgggtcag cttcaggatg ctcctggctc
      481 tctggccgac aatactttcc gaatgcctcc tccttgggaa tgtcaggata gagatagacc
      541 agtggagaca ccaggatatt ggtagcatcc atgatcttat agcccatgat gatttcagca
      601 aatgacatgt tgttcagctg ctgctttgtg tatggttcca cggactggat ctgggtctta
      661 ccgctgatgt ccttctccac ccaagtgaaa gtgacgcctc cttctttgct gctttcactg
      721 aatcttagca ggaaggtgcc tggaggctta gtgctcaaga tggcccgctc ccgctcctta
      781 ctgataaagc ccatgatgta cccttcgttc caaagggcca ggatgtactt tttcacaagg
      841 tcaatgatat tgtccag
//
