LOCUS       sequence-71441-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and HXT7 promoter controlled expression cassettes with 
            phleomycin resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G4-Ph
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(292..481)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(511..540)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(557..576)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(558..576)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      241 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgga tcttcgagcg
      301 tcccaaaacc ttctcaagca aggttttcag tataatgtta catgcgtaca cgcgtctgta
      361 cagaaaaaaa agaaaaattt gaaatataaa taacgttctt aatactaaca taactataaa
      421 aaaataaata gggacctaga cttcaggttg tctaactcct tccttttcgg ttagagcgga
      481 tcttagctag ccgcggtacc aagcttactc gaggtcttct tcggaaatca acttctgttc
      541 catgtcgacg cccgggccct atagtgagtc gtattacgga tccttgtaat taaaacttag
      601 attagattgc tatgctttct ttctaatgag caagaagtaa aaaaagttgt aatagaacaa
      661 gaaaaatgaa actgaaactt gagaaattga agaccgttta ttaacttaaa tatcaatggg
      721 aggtcatcga aagagaaaaa aatcaaaaaa aaaaattttc aagaaaaaga aacgtgataa
      781 aaatttttat tgccttttt
//
