LOCUS       sequence-71444-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with G418 
            resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G2-Km
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     202..219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(291..480)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(510..539)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(556..575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(557..575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      181 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      241 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggat cttcgagcgt
      301 cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac gcgtctgtac
      361 agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa
      421 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat
      481 cttagctagc cgcggtacca agcttactcg aggtcttctt cggaaatcaa cttctgttcc
      541 atgtcgacgc ccgggcccta tagtgagtcg tattacggat ccttgtaatt aaaacttaga
      601 ttagattgct atgctttctt tctaatgagc aagaagtaaa aaaagttgta atagaacaag
      661 aaaaatgaaa ctgaaacttg agaaattgaa gaccgtttat taacttaaat atcaatggga
      721 ggtcatcgaa agagaaaaaa atcaaaaaaa aaaattttca agaaaaagaa acgtgataaa
      781 aatttttatt gcctttttcg
//
