LOCUS       sequence-71445-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with 
            phleomycin resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G2-Ph
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     200..217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(289..478)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(508..537)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(554..573)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(555..573)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
       61 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
      121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
      181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
      241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggatct tcgagcgtcc
      301 caaaaccttc tcaagcaagg ttttcagtat aatgttacat gcgtacacgc gtctgtacag
      361 aaaaaaaaga aaaatttgaa atataaataa cgttcttaat actaacataa ctataaaaaa
      421 ataaataggg acctagactt caggttgtct aactccttcc ttttcggtta gagcggatct
      481 tagctagccg cggtaccaag cttactcgag gtcttcttcg gaaatcaact tctgttccat
      541 gtcgacgccc gggccctata gtgagtcgta ttacggatcc ttgtaattaa aacttagatt
      601 agattgctat gctttctttc taatgagcaa gaagtaaaaa aagttgtaat agaacaagaa
      661 aaatgaaact gaaacttgag aaattgaaga ccgtttatta acttaaatat caatgggagg
      721 tcatcgaaag agaaaaaaat caaaaaaaaa aattttcaag aaaaagaaac gtgataaaaa
      781 tttttattgc ctttttcga
//
