LOCUS       sequence-71447-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and GAL10 promoter controlled expression cassettes with 
            phleomycin resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G3-Ph
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     198..215
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(287..476)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(506..535)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(552..571)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(553..571)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
       61 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      121 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      181 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      241 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggatcttc gagcgtccca
      301 aaaccttctc aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ctgtacagaa
      361 aaaaaagaaa aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat
      421 aaatagggac ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatctta
      481 gctagccgcg gtaccaagct tactcgaggt cttcttcgga aatcaacttc tgttccatgt
      541 cgacgcccgg gccctatagt gagtcgtatt acggatcctt gtaattaaaa cttagattag
      601 attgctatgc tttctttcta atgagcaaga agtaaaaaaa gttgtaatag aacaagaaaa
      661 atgaaactga aacttgagaa attgaagacc gtttattaac ttaaatatca atgggaggtc
      721 atcgaaagag aaaaaaatca aaaaaaaaaa ttttcaagaa aaagaaacgt gataaaaatt
      781 tttattgcct ttttc
//
