LOCUS       sequence-71449-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration at arsB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOarsBCm
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(27..129)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(235..268)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacaac
       61 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
      121 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
      181 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtaggcg cgccgaagtt
      241 cctatacttt ctagagaata ggaacttcgg aataggaact aaggaggata ttcatataga
      301 ccatgattgc atgcggtacc gagctcggat ccactagtag aggataaaac atatggaatt
      361 cctgcagtgc gcagtcgacg cgcaaaaaac cccgcccctg acagggcggg gttttttcgc
      421 tgtttatcca ccggcaccat caccgaggcg tattcgcgaa agccgaggcc aaagaaatca
      481 gcggaaacga tattcaccag gttggagaca ataagcggca ggctggcggt atcggcaatg
      541 aatccggccg ccatcacgaa cgccagcgta gtgcctttac tgaaccctaa agccagcagc
      601 atggcgatga caatcggtgt caaaataagc gccgcgccat cattggcaaa cagggcggca
      661 acggcagcac cgagcaggac aatccaggta aacagcaagc gaccacgacc attaccccag
      721 cgtgagacgt gcagcgccgc ccattcaaaa aagccggact catccagcag caggctgatg
      781 ataacgacgg cgataaacgc agccgtcgcg ttccagacga tattccacac caccggaata
      841 tcac
//
