LOCUS       sequence-71450-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration at lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOlacZCm
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(28..130)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(236..269)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacaa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg
      181 gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtaggc gcgccgaagt
      241 tcctatactt tctagagaat aggaacttcg gaataggaac taaggaggat attcatatag
      301 accatgattg catgcggtac cgagctcgga tccactagta gaggataaaa catatggaat
      361 tcctgcagtg cgcagtcgac gcgcaaaaaa ccccgcccct gacagggcgg ggttttttcg
      421 ctgtttatcc tgcaccatcg tctgctcatc catgacctga ccatgcagag gatgatgctc
      481 gtgacggtta acgcctcgaa tcagcaacgg cttgccgttc agcagcagca gaccattttc
      541 aatccgcacc tcgcggaaac cgacatcgca ggcttctgct tcaatcagcg tgccgtcggc
      601 ggtgtgcagt tcaaccaccg cacgatagag attcgggatt tcggcgctcc acagcgccgg
      661 gttttcgacg ttcagacgta gcgtgacgcg atcggcataa ccaccacgct catcgataat
      721 ttcaccgccg aaaggcgcgg tgccgctggc gacctgcgtt tcaccctgcc acaaagaaac
      781 cgtcacccgc agctcatcgc gcagctcgcc gtacatctga acttctgcct ccagtaccgc
      841 gcggctgaaa tcatcattaa agtgagtggc a
//
