LOCUS       sequence-71451-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration rbsAR locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOrbsARCm
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(33..135)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(241..274)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacaactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
      121 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
      181 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg taggcgcgcc
      241 gaagttccta tactttctag agaataggaa cttcggaata ggaactaagg aggatattca
      301 tatagaccat gattgcatgc ggtaccgagc tcggatccac tagtagagga taaaacatat
      361 ggaattcctg cagtgcgcag tcgacgcgca aaaaaccccg cccctgacag ggcggggttt
      421 tttcgctgtt tcgatcagca ccagcagagc gataagcgat ttctgctcca tcagccacgc
      481 tttcgtgaaa taacggcgac cagagacagt ctgggttgtc atttttttac tcctgattca
      541 cgcgattaag cttgcccaca gcggcagcca ttaacacttc ctgggtggcc tgctcacgag
      601 taaattcccc gctgagatgc ccttcatgca tgacgatgat gcgatcgctc atgcctaata
      661 cttctggcat ctccgatgac accagaatga tgctcaagcc atcggctttg aactggttaa
      721 tcagttgata gatctctttt ttcgcgccga catctacgcc acgggtaggt tcatcaagga
//
