LOCUS       sequence-71454-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid with EGFP expressed from CMV wild-type 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Ferreira JP, Peacock RW, Lawhorn IE, Wang CL
  TITLE     Modulating ectopic gene expression levels by using retroviral 
            vectors equipped with synthetic promoters.
  JOURNAL   Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
  PUBMED    23205156
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Syst Synth 
            Biol."; date: "2011-12"; volume: "5(3-4):131-8. doi"; pages: " 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20"
COMMENT     Sequence Label: pCOCK CMVwt
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(56..75)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(57..75)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    128..152
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(174..377)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(203..223)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        378..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(742..766)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagcacatta accctcacta aagggagcta agcctgcagg ttaataaatt taaatcccta
       61 tagtgagtcg tattattcga accgcgggcc ctctagactc gagcggccgc cactgtgctg
      121 gatatcaacc actttgtaca agaaagctgg gtgatctgac ggttcactaa accagctctg
      181 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      241 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
      301 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
      361 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
      421 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
      481 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
      541 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
      601 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
      661 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
      721 attgattact attaataact aagcctgctt ttttgtacaa acttgttgat atctgcagaa
      781 ttccaccaca ctggactagt ggatccgagc tcggtaccaa gctttttgca aaagcctagt
      841 caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
      901 aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg ta
//
