LOCUS       sequence-71455-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration at arsB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOarsBKm
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(264..297)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
       61 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
      121 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
      181 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
      241 caccccagct tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga
      301 ataggaacta aggaggatat tcatatagac catgattgca tgcggtaccg agctcggatc
      361 cactagtaga ggataaaaca tatggaattc ctgcagtgcg cagtcgacgc gcaaaaaacc
      421 ccgcccctga cagggcgggg ttttttcgct gtttatccac cggcaccatc accgaggcgt
      481 attcgcgaaa gccgaggcca aagaaatcag cggaaacgat attcaccagg ttggagacaa
      541 taagcggcag gctggcggta tcggcaatga atccggccgc catcacgaac gccagcgtag
      601 tgcctttact gaaccctaaa gccagcagca tggcgatgac aatcggtgtc aaaataagcg
      661 ccgcgccatc attggcaaac agggcggcaa cggcagcacc gagcaggaca atccaggtaa
      721 acagcaagcg accacgacca ttaccccagc gtgagacgtg cagcgccgcc cattcaaaaa
      781 agccggactc atccagcagc aggctgatg
//
