LOCUS       sequence-71456-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration at lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOlacZKm
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(267..300)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
       61 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
      121 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
      181 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
      241 gcccacccca gcttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
      301 ggaataggaa ctaaggagga tattcatata gaccatgatt gcatgcggta ccgagctcgg
      361 atccactagt agaggataaa acatatggaa ttcctgcagt gcgcagtcga cgcgcaaaaa
      421 accccgcccc tgacagggcg gggttttttc gctgtttatc ctgcaccatc gtctgctcat
      481 ccatgacctg accatgcaga ggatgatgct cgtgacggtt aacgcctcga atcagcaacg
      541 gcttgccgtt cagcagcagc agaccatttt caatccgcac ctcgcggaaa ccgacatcgc
      601 aggcttctgc ttcaatcagc gtgccgtcgg cggtgtgcag ttcaaccacc gcacgataga
      661 gattcgggat ttcggcgctc cacagcgccg ggttttcgac gttcagacgt agcgtgacgc
      721 gatcggcata accaccacgc tcatcgataa tttcaccgcc gaaaggcgcg gtgccgctgg
      781 cgacctgcgt ttcaccctgc cacaaagaaa ccgtcacccg cagctcatcg cgcagctcgc
      841 cgtacatctg aacttctgcc tccagtaccg cgcggctgaa
//
