LOCUS       sequence-71457-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration rbsAR locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOrbsARKm
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(266..299)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
       61 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
      121 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
      181 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
      241 cccaccccag cttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
      301 gaataggaac taaggaggat attcatatag accatgattg catgcggtac cgagctcgga
      361 tccactagta gaggataaaa catatggaat tcctgcagtg cgcagtcgac gcgcaaaaaa
      421 ccccgcccct gacagggcgg ggttttttcg ctgtttcgat cagcaccagc agagcgataa
      481 gcgatttctg ctccatcagc cacgctttcg tgaaataacg gcgaccagag acagtctggg
      541 ttgtcatttt tttactcctg attcacgcga ttaagcttgc ccacagcggc agccattaac
      601 acttcctggg tggcctgctc acgagtaaat tccccgctga gatgcccttc atgcatgacg
      661 atgatgcgat cgctcatgcc taatacttct ggcatctccg atgacaccag aatgatgctc
      721 aagccatcgg ctttgaactg gttaatcagt tgatagatct cttttttcgc gccgacatct
      781 acgccacggg taggttcatc aaggatcaac actttggggc gtgtcat
//
