LOCUS       sequence-71462-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid with EGFP expressed from synthetic 
            promoter (CMVwt CAAT box mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Ferreira JP, Peacock RW, Lawhorn IE, Wang CL
  TITLE     Modulating ectopic gene expression levels by using retroviral 
            vectors equipped with synthetic promoters.
  JOURNAL   Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
  PUBMED    23205156
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Syst Synth 
            Biol."; date: "2011-12"; volume: "5(3-4):131-8. doi"; pages: " 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20"
COMMENT     Sequence Label: pCOCK CM-C18
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(63..82)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(64..82)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    135..159
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(181..383)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        384..687
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(748..772)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attattccag cacattaacc ctcactaaag ggagctaagc ctgcaggtta ataaatttaa
       61 atccctatag tgagtcgtat tattcgaacc gcgggccctc tagactcgag cggccgccac
      121 tgtgctggat atcaaccact ttgtacaaga aagctgggtg atctgacggt tcactaaacc
      181 agctctgcta tatagacctc ccaccgtaca cgcctaccgc ccacttgcgt caatggggcg
      241 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
      301 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
      361 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
      421 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
      481 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
      541 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
      601 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
      661 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
      721 cccgtaattg attactatta ataactaagc ctgctttttt gtacaaactt gttgatatct
      781 gcagaattcc accacactgg actagtggat ccgagctcgg taccaagctt tttgcaaaag
      841 cctagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa
      901 acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc
      961 ttaaattttg ctcgcgcact actcagcgac ctcca
//
