LOCUS       sequence-71470-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mitotrap in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Robinson MS, Sahlender DA, Foster SD
  TITLE     Rapid inactivation of proteins by rapamycin-induced rerouting to 
            mitochondria.
  JOURNAL   Dev Cell. 2010 Feb 16;18(2):324-31. doi: 
            10.1016/j.devcel.2009.12.015.
  PUBMED    20159602
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2009.12"; journalName: "Dev Cell"; date: 
            "2010-02-16- 16"; volume: "18"; issue: "2"; pages: "324-31"
COMMENT     Sequence Label: pEYFP-Mitotrap
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      169..178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(175..177,178..180,181..888)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLKPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(220..241)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(481..500)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     828..849
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             892..918
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcgc caccatgaag
       61 agcttcatta caaggaacaa gacagccatt ttggcaaccg ttgctgctac aggtactgcc
      121 atcggtgcct actattatta caaccaattg caacaggatc caccggtcgc caccatggtg
      181 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      241 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      301 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      361 accaccttcg gctacggcct gatgtgcttc gcccgctacc ccgaccacat gaagcagcac
      421 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      481 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      541 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      601 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      661 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      721 taccagcaga acacccccat cggcgacggc cccgtgctga agcccgacaa ccactacctg
      781 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      841 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacac ttacccctac
      901 gacgtgcccg actacgcagg ctccgcacga gtggccatcc tctggcatga
//
