LOCUS       sequence-71473-e        2134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Goldstein JC, Waterhouse NJ, Juin P, Evan GI, Green DR
  TITLE     The coordinate release of cytochrome c during apoptosis is rapid, 
            complete and kinetically invariant.
  JOURNAL   Nat Cell Biol. 2000 Mar;2(3):156-62.
  PUBMED    10707086
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2000-03"; volume: "2(3)"; pages: "156-62"
COMMENT     Sequence Label: pBabe(LTR)-Cytochrome C-GFP
FEATURES             Location/Qualifiers
     source          1..2134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     314..336
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     352..368
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      734..743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(740..742,743..745,746..1459)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     XNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(785..806)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1046..1065)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1393..1414
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1534..1554)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1539..1868
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1719..1854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1878..1897)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
       61 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
      121 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
      181 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
      241 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
      301 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
      361 gccctcactc cttctctagg cgccggccgg atctcgacct aatatgggtg atgttgaaaa
      421 aggcaagaag atttttgttc agaagtgtgc ccagtgccac actgtggaaa agggaggcaa
      481 gcataagact ggaccaaatc tccacggtct gttcgggcgg aagacaggcc aggctgctgg
      541 attctcttac acagatgcca acaagaacaa aggcatcacc tggggagagg ataccctgat
      601 ggagtatttg gagaatccca aaaagtacat ccctggaaca aaaatgatct tcgctggaat
      661 taagaagaag ggagaaaggg cagacctaat agcttatctt aaaaaggcta ctaatgagaa
      721 agatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      781 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt cyggcgaggg
      841 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      901 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      961 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1021 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1081 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1141 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1201 ggccgacaag cagaagaacg gcatcaaggy gaacttcaag atccgccaca acatcgagga
     1261 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1321 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1381 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1441 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
     1501 gtaggaattc gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa
     1561 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     1621 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     1681 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1741 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1801 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1861 tttgcaaaaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     1921 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     1981 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     2041 cgcgcgtcgg gctcgacatc ggcaaggtgt ggggtcgcgg acgacggcgc cgcggtggcg
     2101 gtctggacca cgccggagag cgtcgaagcg gggg
//
