LOCUS       sequence-71519-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid with EGFP expressed from synthetic 
            promoter (random mutagenesis of CMVwt 3' end)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Ferreira JP, Peacock RW, Lawhorn IE, Wang CL
  TITLE     Modulating ectopic gene expression levels by using retroviral 
            vectors equipped with synthetic promoters.
  JOURNAL   Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
  PUBMED    23205156
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Syst Synth 
            Biol."; date: "2011-12"; volume: "5(3-4):131-8. doi"; pages: " 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20"
COMMENT     Sequence Label: pCOCK CM-R03
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(54..73)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(55..73)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        376..679
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(740..764)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcacattaac cctcactaaa gggagctaag cctgcaggtt aataaattta aatccctata
       61 gtgagtcgta ttattcgaac cgcgggccct ctagactcga gcggccgcca ctgtgctgga
      121 tatcaaccac tttgtacaag aaagctgggt catcggtcgg tacactcttc ctggtccacg
      181 tatctatggc acgtacggta cccgccgacc gcccatttgc gtcaatgggg cggagttgtt
      241 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
      301 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
      361 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
      421 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
      481 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
      541 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
      601 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
      661 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
      721 tgattactat taataactaa gcctgctttt ttgtacaaac ttgttgatat ctgcagaatt
      781 ccaccacact ggactagtgg atccgagctc ggtaccaagc tttttgcaaa agcctagtca
      841 ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa
      901 ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaa
//
