LOCUS       sequence-7152-ex        2160 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Le Y, Miller JL, Sauer B
  TITLE     GFPcre fusion vectors with enhanced expression.
  JOURNAL   Anal Biochem. 1999 Jun 1. 270(2):334-6.
  PUBMED    10334853
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 1999 Jun 1. 270(2):334-6."
COMMENT     Sequence Label: pBS505 EF1alpha-EGFPcre*
FEATURES             Location/Qualifiers
     source          1..2160
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          340..543
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     500..520
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      576..585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(582..584,585..587,588..1298)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(582..584,585..587,588..1298)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(888..907)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1235..1256
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
       61 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
      121 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
      181 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
      241 gagtcaccca cacaaaggaa aagggccttt ccgtcgtcag ccgtcgcttc atgtgactcc
      301 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
      361 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
      421 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
      481 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
      541 caggtgtcgt gaggaattct ctagcgctac cggtcgccac catggtgagc aagggcgagg
      601 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      661 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      721 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      781 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      841 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      901 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      961 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1021 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1081 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1141 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1201 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1261 ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc
     1321 aagcttcgaa ttctgcagtc gacggagaaa gctcaggctc tggctcagag tctgactcca
     1381 tggccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg
     1441 atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata
     1501 cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc
     1561 ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc
     1621 gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc
     1681 ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc
     1741 gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg
     1801 atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat atacgtaatc
     1861 tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca
     1921 gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga
     1981 aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg
     2041 tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc
     2101 gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc
//
