LOCUS       sequence-71521-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length RAMA ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RAMA-bio
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     205..224
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    223..278
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 agaacccctt tgtgtttggt ccccccccgt gtcacatgtg gaacagggcc cagttggcaa
       61 gttgtaccaa ccaactgaag ggattacatg cactgccccg cgaagaaggg gcagagatgt
      121 cgtagtcagg tttagttcgt ccggggcggg gatcgatcct ctagctagag atctccgagg
      181 gatctcgacc aaatgatttg ccctcccata tgtccttccg agtgagagac acaaaaaatt
      241 ccaacacact attgcaatga aaataaattt cctttattag ccagaggtcg aggtcggggg
      301 atccttaatt aatcacgtgt ctagacttaa cgatgattcc acaccatttt ctgtgcatcc
      361 agaatatgat gcagtgatcc ggaattcaac cctttggata aaacctggat cttggagtcc
      421 atcttgacct cttcaccttc actcagtaga cattgccaca cccctgcttc aggggcctgc
      481 acttgaatca ctttctcctg cctggagacc ctggcctcct gattctcctg cttcaagatc
      541 agtctcatct tgggtgaggt gggtcccatc acctcacagg tcaaagtgtt gctgtcgggc
      601 tgagtcactt tcatcaccac caggttcact tcctgataca gtatccctct gtccagagtc
      661 agggtcaggt tgccagaacc agcaaactga agggagacct ggggtatctg aagggtgagt
      721 gggagcgttt cggacagctg gaacttgggg ttgctagtag acttctgcac agacaccttt
      781 tggttcttta gggagaaggt gatccaggac tgggaagaag gagccttctc tgccttccat
      841 ctcaactctc cctgcaggct ttcctctcca aggttgagtg ggaaggagaa ctccgctgac
      901 tccccctcac tcttataggc cgtgatgga
//
