LOCUS       sequence-71523-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid with EGFP expressed from synthetic 
            promoter (random mutagenesis of CMVwt 3' end)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Ferreira JP, Peacock RW, Lawhorn IE, Wang CL
  TITLE     Modulating ectopic gene expression levels by using retroviral 
            vectors equipped with synthetic promoters.
  JOURNAL   Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
  PUBMED    23205156
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Syst Synth 
            Biol."; date: "2011-12"; volume: "5(3-4):131-8. doi"; pages: " 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20"
COMMENT     Sequence Label: pCOCK CM-R05
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(54..73)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(55..73)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        375..678
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(738..762)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcacattaac cctcactaaa gggagctaag cctgcaggtt aataaattta aatccctata
       61 gtgagtcgta ttattcgaac cgcgggccct ctagactcga gcggccgcca ctgtgctgga
      121 tatcaaccac tttgtacaag aaagctgggt gatatgtcag gtcaaagtat cagctcggct
      181 gctctagacc tctgatgtct acccctgccg ccaagttgcg tcaatgggac ggagttgtta
      241 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
      301 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      361 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      421 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
      481 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
      541 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
      601 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      661 accgtaagtt atgtaacgcg gaactccata tatgggctac gaactaatga ccccgtaatt
      721 gattactatt aatactaagc ctgctttttt gtacaaactt gttgatatct gcagaattcc
      781 accacactgg actagtggat ccgagctcgg taccaagctt tttgcaaaag cctagtcaat
      841 aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc
      901 cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaattttg
      961 ctcgcgcact actcagcgac ctccaacaca
//
