LOCUS       sequence-71533-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length PTRAMP 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: PTRAMP-bio
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..228
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    227..282
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg
       61 gcaagttgta ccaaccaact gaagggatta catgcactgc cccgcgaaga aggggcagag
      121 atgtcgtagt caggtttagt tcgtccgggg cggggatcga tcctctagct agagatctcc
      181 gagggatctc gaccaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
      241 aattccaaca cactattgca atgaaaataa atttccttta ttagccagag gtcgaggtcg
      301 ggggatcctt aattaatcac gtgtctagac ttaacgatga ttccacacca ttttctgtgc
      361 atccagaata tgatgcagtg atccggaatt caaccctttg gataaaacct ggatcttgga
      421 gtccatcttg acctcttcac cttcactcag tagacattgc cacacccctg cttcaggggc
      481 ctgcacttga atcactttct cctgcctgga gaccctggcc tcctgattct cctgcttcaa
      541 gatcagtctc atcttgggtg aggtgggtcc catcacctca caggtcaaag tgttgctgtc
      601 gggctgagtc actttcatca ccaccaggtt cacttcctga tacagtatcc ctctgtccag
      661 agtcagggtc aggttgccag aaccagcaaa ctgaagggag acctggggta tctgaagggt
      721 gagtgggagc gtttcggaca gctggaactt ggggttgcta gtagacttct gcacagacac
      781 cttttggttc tttagggaga aggtgatcca ggactgggaa gaaggagcct tctctgcctt
      841 ccatctcaac tctccctgca ggctttcctc tccaaggttg agtgggaagg a
//
