LOCUS       sequence-71534-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET E. coli expression vector with BioBrick
            po...
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      60..87
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        113..131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      223..269
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgttgtttg tcggtgagtc cttccagtct gggcgaaagc gcggtcgatc aaattaagca
       61 gaaggccatc ctgacggatg gcctttttgc gtttctatcg atcccgcgaa attaatacga
      121 ctcactatag gcccctctag aaataatttt gtttaacttt aagctttaag aaggagatat
      181 agatatccca actccataag tggctgctgc caccgctgag catagcataa ccccttgggg
      241 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatca agtcccgaag
      301 tagctgtcag actgctggcg accgccgcaa ctagtggcgt catgcgtgcc gtatggtctt
      361 tcgcctgtag acgccctaca agccgtacta aagtggaaag acccgcaaaa ggtatcgcga
      421 tcgccctgca gggaactata tccgactagt agcccgccta atgagcgggc ttttttttaa
      481 ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      541 accctgataa atgcttcgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      601 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctcgtgaa
      661 agtaaaagac gcagaggacc aattgggggc acgagtggga tacatagaac tggacttgaa
      721 tagcggtaaa atccttgaga gttttcgccc tgaagagcgt tttccaatga tgagcacttt
      781 caaagttctg ctatgtggag cagtattatc ccgtgtagat gcggggcaag agcaactcgg
      841 acgacgaata cactattcgc agaatgactt ggttgaatac tccccagtga cagaaaagca
      901 ccttacggac ggaatgacgg taagagaatt atgtagtgcc gccataacga tgagtgataa
      961 cactgcggcg aacttacttc tgacaaccat cggtggaccg aaggaattaa ccgctttttt
     1021 gcacaatatg ggagaccatg taactcgcct tgaccgttgg gaaccagaac tgaatgaagc
     1081 c
//
