LOCUS       sequence-71542-e        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 TEV expression vector with BioBrick
            p...
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      61..88
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        114..132
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             149..171
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             190..207
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             208..228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      270..316
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgttgttt gtcggtgagt ccttccagtc tgggcgaaag cgcggtcgat caaattaagc
       61 agaaggccat cctgacggat ggcctttttg cgtttctatc gatcccgcga aattaatacg
      121 actcactata ggcccctcta gaaataattt tgtttaactt taagaaggag atataccatg
      181 ggttcttctc accatcacca tcaccatgaa aacctgtact tccaatccaa tattggaagt
      241 ggataactgg ctgctgccac cgctgagcat agcataaccc cttggggcct ctaaacgggt
      301 cttgaggggt tttttgctga aaggaggaac tatatcaagt cccgaagtag ctgtcagact
      361 gctggcgacc gccgcaacta gtggcgtcat gcgtgccgta tggtctttcg cctgtagacg
      421 ccctacaagc cgtactaaag tggaaagacc cgcaaaaggt atcgcgatcg ccctgcaggg
      481 aactatatcc gactagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt
      541 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      601 cttcgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      661 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tcgtgaaagt aaaagacgca
      721 gaggaccaat tgggggcacg agtgggatac atagaactgg acttgaatag cggtaaaatc
      781 cttgagagtt ttcgccctga agagcgtttt ccaatgatga gcactttcaa agttctgcta
      841 tgtggagcag tattatcccg tgtagatgcg gggcaagagc aactcggacg acgaatacac
      901 tattcgcaga atgacttggt tgaatactcc ccagtgacag aaaagcacct tacggacgga
      961 atgacggtaa gagaattatg tagtgccgcc ataacgatga gtgataacac tgcggcgaac
     1021 ttacttctga caaccatcgg tggaccgaag gaattaaccg c
//
