LOCUS       sequence-71547-e        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 MBP TEV expression vector with BioBri...
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..22
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      64..110
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaaaacctg tacttccaat ccaatattgg aagtggataa ctggctgctg ccaccgctga
       61 gcatagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      121 gaactatatc aagtcccgaa gtagctgtca gactgctggc gaccgccgca actagtggcg
      181 tcatgcgtgc cgtatggtct ttcgcctgta gacgccctac aagccgtact aaagtggaaa
      241 gacccgcaaa aggtatcgcg atcgccctgc agggaactat atccgactag tagcccgcct
      301 aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac attcaaatat
      361 gtatccgctc atgagacaat aaccctgata aatgcttcga aaaaggaaga gtatgagtat
      421 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      481 tcacccagaa acgctcgtga aagtaaaaga cgcagaggac caattggggg cacgagtggg
      541 atacatagaa ctggacttga atagcggtaa aatccttgag agttttcgcc ctgaagagcg
      601 ttttccaatg atgagcactt tcaaagttct gctatgtgga gcagtattat cccgtgtaga
      661 tgcggggcaa gagcaactcg gacgacgaat acactattcg cagaatgact tggttgaata
      721 ctccccagtg acagaaaagc accttacgga cggaatgacg gtaagagaat tatgtagtgc
      781 cgccataacg atgagtgata acactgcggc gaacttactt ctgacaacca tcggtggacc
      841 gaaggaatta accgcttttt tgcacaatat gggagaccat gtaactcgcc ttgaccgttg
      901 ggaaccagaa ctgaatgaag ccataccaaa cgacgagcga gacaccacaa tgcctgcggc
      961 aatggcaaca acattacgca aactattaac tggcgaacta cttactctgg cttcacggca
     1021 acaattaata gactggcttg aagcggataa agttgcagga ccactactgc gttcggcact
     1081 tcctgctggc tggtttattg ctga
//
