LOCUS       sequence-71549-e        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Mocr TEV expression vector with BioBr...
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      61..88
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        114..132
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             149..171
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             190..207
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             577..597
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      639..685
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgttgttt gtcggtgagt ccttccagtc tgggcgaaag cgcggtcgat caaattaagc
       61 agaaggccat cctgacggat ggcctttttg cgtttctatc gatcccgcga aattaatacg
      121 actcactata ggcccctcta gaaataattt tgtttaactt taagaaggag atataccatg
      181 ggttcttctc accatcacca tcaccatggt tcttctatga gcaacatgac atacaataat
      241 gttttcgacc acgcgtacga aatgcttaag gagaacatcc gttatgatga catacgggat
      301 acagatgacc ttcacgacgc aattcatatg gccgccgata atgccgtccc tcactattat
      361 gccgatattc ggtcagttat ggcatctgaa ggtattgatc tggaatttga ggatagtggc
      421 ctcatgccag atacgaagga cgacatcaga attctgcaag cgcggatcta cgaacaactt
      481 accatcgatt tatgggaaga tgcggaagat ctcctgaatg aatacctgga agaagtggag
      541 gagtatgagg aagatgaaga ggggacgggg atcgaggaaa acctgtactt ccaatccaat
      601 attggaagtg gataactggc tgctgccacc gctgagcata gcataacccc ttggggcctc
      661 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatcaagtc ccgaagtagc
      721 tgtcagactg ctggcgaccg ccgcaactag tggcgtcatg cgtgccgtat ggtctttcgc
      781 ctgtagacgc cctacaagcc gtactaaagt ggaaagaccc gcaaaaggta tcgcgatcgc
      841 cctgcaggga actatatccg actagtagcc cgcctaatga gcgggctttt ttttaattcc
      901 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      961 tgataaatgc ttcgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1021 cccttttttg cggcattttg ccttcctgtt tttgctcacc
//
