LOCUS       sequence-7155-ex        4891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Zuger S, Iwai H
  TITLE     Intein-based biosynthetic incorporation of unlabeled protein tags 
            into isotopically labeled proteins for NMR studies.
  JOURNAL   Nat Biotechnol. 2005 Jun . 23(6):736-40.
  PUBMED    15908942
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2005 Jun . 23(6):736-40."
COMMENT     Sequence Label: pSZBAD1PG
FEATURES             Location/Qualifiers
     source          1..4891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(343..360)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      512..598
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      690..717
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        735..826
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(827..895,896..1687)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1045..1064)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1729..2184
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1816..1835)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2026..2047
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      2295..2883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2784..2803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3037..3054
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3069..3209
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3135..3154)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3295..3317
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3456..3475
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(3658..4536)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4563..4847
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4770..4789
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             join(4874..4891,1..5)
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggagatatac atatggttaa agttatcggt cgtcgatccc tgggtgtgca acgcatcttt
       61 gatatcggtc tgccgcaaga ccataatttt ctgctggcta atggtgctat cgccgccaac
      121 tgttttaaca aaggtaccca gtacaaactt atcctgaacg gtaaaaccct gaaaggtgaa
      181 accaccaccg aagctgttga cgctgctacc gcggaaaaag ttttcaaaca gtacgctaac
      241 gacaacggtg ttgacggtga atggacctac gacgacgcta ccaaaaccta caccgtaacg
      301 gaataagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
      361 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
      421 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
      481 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtgcaaaga
      541 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      601 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
      661 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      721 gtttctacaa actctttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      781 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      841 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      901 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      961 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1021 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     1081 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1141 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1201 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1261 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1321 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     1381 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1441 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1501 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1561 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1621 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1681 ttggtaactg tcagaccaag tttactcata tatactttag attgatttac gcgccctgta
     1741 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1801 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1861 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1921 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     1981 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2041 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     2101 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2161 acaaaatatt aacgtttaca atttaaaagg atctaggtga agatcctttt tgataatctc
     2221 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2281 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2341 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2401 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2461 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2521 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2581 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2641 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     2701 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2761 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2821 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2881 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2941 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3001 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3061 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccggata
     3121 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     3181 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     3241 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3301 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag caaggagatg
     3361 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     3421 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     3481 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctaa
     3541 ttctcatgtt tgacagctta tcatcgatgc ataatgtgcc tgtcaaatgg acgaagcagg
     3601 gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg ttaccaatta
     3661 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg
     3721 gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac
     3781 attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct
     3841 gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg
     3901 tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct
     3961 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg
     4021 atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat
     4081 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag
     4141 gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat
     4201 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg
     4261 ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag
     4321 caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc
     4381 gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc
     4441 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta
     4501 tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag
     4561 agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct
     4621 cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac
     4681 caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat
     4741 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc
     4801 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg
     4861 ggctagaaat aattttgttt aactttaaga a
//
