LOCUS       sequence-71551-e        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET MBP TEV expression vector with BrioBrick
            p...
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      61..88
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        114..132
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             149..171
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tctgttgttt gtcggtgagt ccttccagtc tgggcgaaag cgcggtcgat caaattaagc
       61 agaaggccat cctgacggat ggcctttttg cgtttctatc gatcccgcga aattaatacg
      121 actcactata ggcccctcta gaaataattt tgtttaactt taagaaggag atataccatg
      181 ggttcttctg gttctaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa
      241 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
      301 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
      361 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
      421 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
      481 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg
      541 atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg
      601 gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac
      661 ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag
      721 tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg
      781 gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct
      841 gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc
      901 gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc
      961 aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg
     1021 gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaa
//
